Let's say you have made a dilution where you put 10mL sample and you added 40mL water (this is the diluent). You have 1 part of sample (the blue boxes above) for 4 parts of water (the red boxes above). If you express this as S:D, it's going to be 1:4. If you express this as S:T, you're going to have 1:5 (as you have 1+4=5 parts in total).

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